![]() ![]() Analysis of IC-MeOH permitted the identification of 10 compounds already reported for their anticancer activity, epicatechin, curcumin, (-)-yatein, caffeic acid, myricetin, jatrorrhizine, harmaline, cinnamaldehyde, dobutamine, and syringin. The plant extract was also found to induce a concentration- and time-dependent apoptosis and cell cycle arrest in the G0/G1 phase (IC 50 value) in CaSki cell line. Our findings revealed that IC-MeOH inhibited the proliferation of HeLa and CaSki cells. Based on high-mass accuracy, spectral data, and previous reports, tentative compound identifications were assigned. The ultrahigh-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) analysis was used for the metabolites profiling of IC-MeOH. Cell apoptosis and cell cycle were measured by flow cytometry-based fluorescence-activated cell sorting. The water-soluble tetrazolium-1 and colony formation assays were used to check the proliferation ability of the cells. The present study was designed to investigate the anticancer activity of the methanolic root extract of I. Imperata cylindrica is traditionally used to cure several diseases including cancer, wounds, and hypertension.
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